• br Materials and methods br Acknowledgments We thank Dr

  2018-10-20

  

  Materials and methods Acknowledgments We thank Dr. Keisuke Okita and Prof. Shinya Yamanaka for providing the reprogramming plasmids. Additionally, we would like to thank Tina Christoffersen and Birgit Kassow for skillful technical assistance. We acknowledge SHARE\'s Cross Faculty PhD Initiativ

• br Materials and methods br

  2018-10-20

  

  Materials and methods Results and discussion To generate an OKSM mouse model with higher reprogramming efficiency we exchanged the m2rtTA for the more efficient rtTA3 (Das et al., 2004). We used founder animals which can be found commercially available at the Jackson Institute and applied a br

• The transgenic iPSCs differentiate into embryoid

  2018-10-20

  

  The transgenic iPSCs differentiate into embryoid bodies (EBs) containing spontaneously beating EGFP-positive areas, which first appear on day 7–9 of differentiation (Fig. 2A). After 7days of puromycin treatment, nearly all non-cardiomyocytes in EBs are eliminated and the resistant p38 inhibitor sur

• br Materials and methods br Author disclosure

  2018-10-20

  

  Materials and methods Author disclosure statement Acknowledgments This study was supported by fund from National Medical Research Council (NMRC) of Singapore, the Translational Clinical Research (TCR) award (NMRC/TCR/013-NNI/2014) and STaR award (NMRC/STaR/014/2013). We thank Ms. Fiona Seti

• Here we used iPSCs to generate cardiomyocytes

  2018-10-20

  

  Here, we used iPSCs to generate cardiomyocytes by reprogramming skin fibroblasts from two patients carrying dissimilar LQTS mutations. Associated with LQT2, HERG-A422T produces trafficking-defective HERG K+ Calcitriol (Kv 11.1) that decrease the rapidly activating delayed rectifier current (IKr), w

• br Results br Discussion Adrenergic

  2018-10-20

  

  Results Discussion α-Adrenergic receptors play a critical role in the regulation of cellular growth pathways, including hypertrophy and proliferation. G protein-coupled α1ARs are the predominant α-adrenergic subtype in the myocardium of most species (Brückner et al., 1985), and catecholamine r

• To address this need we have developed a functional

  2018-10-20

  

  To address this need, we have developed a functional imaging approach for CSC identification. The stem cell phenotype in embryonic stem calcium channel blockers (ESCs) is maintained by a central triad of master transcriptional regulators, OCT4, SOX2, and NANOG, which promote stemness by upregulatin

• Epithelial stem cells in the mammary microenvironment may be

  2018-10-20

  

  Epithelial stem arginase inhibitor in the mammary microenvironment may be exposed to abnormally high levels of BMP2 over a prolonged period. Simultaneously, epithelial cells, including stem cells, may accumulate genetic alterations that increase their susceptibility to transforming agents, such as a

• br Conclusions Whilst it is clear that RNA binding

  2018-10-20

  

  Conclusions Whilst it is clear that RNA-binding proteins play crucial roles in regulating oogenesis, understanding the functions of these proteins relies on identifying the RNA targets they bind and regulate. Given the relative promiscuity of RNA-binding proteins, it seems likely that the fertili

• The seemingly shared ability of independently evolved Extavo

  2018-10-20

  

  The seemingly shared ability of independently evolved (Extavour and Akam, 2003; Ewen-Campen et al., 2010) maternal germ plasm regulators to engage multiple proteins and RNA partners provide new insight and testable models for mechanisms by which germ plasm assembly, mitochondrial recruitment and Bal

• br Results br Discussion The self renewal

  2018-10-20

  

  Results Discussion The self-renewal capacity of erythroid progenitors is an important component of current protocols to generate cultured RBCs for potential therapeutic purposes. However, a major obstacle in generating the 2.5 × 1012 RBCs that constitutes a single unit of blood is the limited

• In this work we developed

  2018-10-20

  

  In this work, we developed multiple tools to interrogate this autocrine and paracrine signaling loop involving α-5 laminin production by hPSCs. The use of the inducible short hairpin lines allowed us to control the dynamics of α-5 laminin production in a reversible manner. However, the interpretatio

• Several approaches exist to prevent

  2018-10-20

  

  Several approaches exist to prevent allogeneic cell transplantation-related immune rejection. One is immune suppression therapy using a combination of several different types of immunosuppressants. Others are the use of major histocompatibility complex (MHC)-matched donor cells to reduce immunogenic

• aldehyde dehydrogenase inhibitor We speculate that ROS reduc

  2018-10-20

  

  We speculate that ROS reduction has an important, but not exclusive, regulatory role in the cAMP-mediated maintenance of HSC-like cells. Moreover, cAMP-mediated ROS/p38 reduction and increased CXCR4 receptor expression provide a correlational benefit to HSC-like cells, as low ROS levels specifically

• mSSCs can be maintained and genetically manipulated

  2018-10-20

  

  mSSCs can be maintained and genetically manipulated in vitro over extensive periods, providing a valuable resource for in vitro studies of meiosis and post-meiotic development (Kanatsu-Shinohara et al., 2003; Kubota et al., 2004). In the past decade, this culture system has been used to identify key

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