• A recent study on hair cell differentiation in

  2018-10-22

  

  A recent study on hair cell differentiation in adult animals with hair cell loss after Notch inhibition did not identify the supporting ion channels that responded (Mizutari et al., 2013). The results we describe here are surprising, because we did not expect that the transdifferentiation of suppor

• The generation of hESCs involves numerous reagents including

  2018-10-22

  

  The generation of hESCs involves numerous reagents, including growth factors, small molecules, and media. To avoid infection by animal-sourced components, mouse feeder daminozide can be substituted by human fibroblasts (Ellerstrom et al., 2006; Genbacev et al., 2005). Chemically defined hESC cultur

• br Results br Discussion Culturing hFK in mNPEM instantly

  2018-10-22

  

  Results Discussion Culturing hFK in mNPEM instantly showed morphological heterogeneity, including specific undifferentiated stem cell niches and differentiating epithelial cells, which could be further exploited to show an EpCAM expression gradient (EpCAM−, EpCAMdim, EpCAMbright) within the NC

• KU-60019 br Materials and methods br Results

  2018-10-20

  

  Materials and methods Results Discussion In vitro, hBMSCs are capable of differentiating into a variety of cell types including osteo-, chondro- and adipocytes (Pittenger et al., 1999). This fuelled the idea of their use for regenerative cellular therapy of a wide range of human pathologies

• Finally we observed decreased leukocyte influx in

  2018-10-20

  

  Finally, we observed decreased leukocyte influx in exosome treated animals. It is likely that the reduced influx is secondary to the reduced cardiac injury after exosome treatment, since leukocyte influx did not differ between the groups at 1h reperfusion. In addition, we found that the reduced infa

• br Materials and methods br Verification

  2018-10-20

  

  Materials and methods Verification of karyotype Acknowledgments This project was funded by the Department of Biotechnology, Government of India (grant no. BT/IN/Denmark/31/MSI/2013) and the Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore. Resource Table: Resource d

• br Materials and methods br Acknowledgments We thank Dr

  2018-10-20

  

  Materials and methods Acknowledgments We thank Dr. Keisuke Okita and Prof. Shinya Yamanaka for providing the reprogramming plasmids. Additionally, we would like to thank Tina Christoffersen and Birgit Kassow for skillful technical assistance. We acknowledge SHARE\'s Cross Faculty PhD Initiativ

• br Materials and methods br

  2018-10-20

  

  Materials and methods Results and discussion To generate an OKSM mouse model with higher reprogramming efficiency we exchanged the m2rtTA for the more efficient rtTA3 (Das et al., 2004). We used founder animals which can be found commercially available at the Jackson Institute and applied a br

• The transgenic iPSCs differentiate into embryoid

  2018-10-20

  

  The transgenic iPSCs differentiate into embryoid bodies (EBs) containing spontaneously beating EGFP-positive areas, which first appear on day 7–9 of differentiation (Fig. 2A). After 7days of puromycin treatment, nearly all non-cardiomyocytes in EBs are eliminated and the resistant p38 inhibitor sur

• br Materials and methods br Author disclosure

  2018-10-20

  

  Materials and methods Author disclosure statement Acknowledgments This study was supported by fund from National Medical Research Council (NMRC) of Singapore, the Translational Clinical Research (TCR) award (NMRC/TCR/013-NNI/2014) and STaR award (NMRC/STaR/014/2013). We thank Ms. Fiona Seti

• Here we used iPSCs to generate cardiomyocytes

  2018-10-20

  

  Here, we used iPSCs to generate cardiomyocytes by reprogramming skin fibroblasts from two patients carrying dissimilar LQTS mutations. Associated with LQT2, HERG-A422T produces trafficking-defective HERG K+ Calcitriol (Kv 11.1) that decrease the rapidly activating delayed rectifier current (IKr), w

• br Results br Discussion Adrenergic

  2018-10-20

  

  Results Discussion α-Adrenergic receptors play a critical role in the regulation of cellular growth pathways, including hypertrophy and proliferation. G protein-coupled α1ARs are the predominant α-adrenergic subtype in the myocardium of most species (Brückner et al., 1985), and catecholamine r

• To address this need we have developed a functional

  2018-10-20

  

  To address this need, we have developed a functional imaging approach for CSC identification. The stem cell phenotype in embryonic stem calcium channel blockers (ESCs) is maintained by a central triad of master transcriptional regulators, OCT4, SOX2, and NANOG, which promote stemness by upregulatin

• Epithelial stem cells in the mammary microenvironment may be

  2018-10-20

  

  Epithelial stem arginase inhibitor in the mammary microenvironment may be exposed to abnormally high levels of BMP2 over a prolonged period. Simultaneously, epithelial cells, including stem cells, may accumulate genetic alterations that increase their susceptibility to transforming agents, such as a

• br Conclusions Whilst it is clear that RNA binding

  2018-10-20

  

  Conclusions Whilst it is clear that RNA-binding proteins play crucial roles in regulating oogenesis, understanding the functions of these proteins relies on identifying the RNA targets they bind and regulate. Given the relative promiscuity of RNA-binding proteins, it seems likely that the fertili

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